DNA Microarrays and Gene Expression: From Experiments to Data Analysis and Modeling

by Pierre Baldi, G. Wesley Hatfield, Wesley G. Hatfield

Publisher: Cambridge University Press
ISBN: 0521176352

Check price @ amazon.com , amazon.ca , amazon.co.uk

Book Info

An interdisciplinary introduction to DNA arrays is essential reading for both biology and computer science researchers wanting to take advantage of this powerful new technology.

Massive data acquisition technologies such as genome sequencing, high-throughput drug screening, and DNA arrays are in the process of revolutionizing biology and medicine. This concise, user-friendly and interdisciplinary guide to DNA microarray technology is an introduction and a reference for both biologists and computational scientists. The authors describe the underlying technologies and offer an awareness of the "noise" and pitfalls present in the data generated. They also provide an idea of the different data mining techniques and algorithms that are available to interpret data, and the advantages and disadvantages of each in differing situations.

Table of Contents

1. A brief history of genomics
2. DNA array formats
3. DNA array readout methods
4. Gene expression profiling experiments: problems, pitfalls and solutions
5. Statistical analysis of array data: inferring changes
6. Statistical analysis of array data: dimensionality reduction, clustering, and regulatory regions
7. Survey of current DNA array applications
8. Systems biology: overview of regulatory, metabolic and signaling networks.

Customer Reviews

This book has a good balance between experimental and computational methods. It provides a description of DNA microarray technologies, experimental protocols, and the multiple sources of noise and variability. The book contains an insightful overview of the computational issues and available algorithms for data analysis from differential expression, to dimensionality reduction and visualization (e.g. PCA), to clustering (e.g. hierarchical). New methods are described to gether with a good overview of available software, data bases, web sites, and other resources, as well as several "walk through" examples. I particularly enjoyed the last chapter on Systems Biology.

"Very complete : covers both the experimental and the computational methods with specific examples. Written by two top scientists who have worked hard at complementing each other's strengths. I particularly enjoyed the last chapter on Systems Biology which provides a masterful overview of current resaerch trends."